Worked example
200 observed cells across four large squares, with a dilution factor of 2, corresponds to 1,000,000 cells/mL.
Assumptions and limitations
Only for the stated chamber geometry. Count consistently at boundaries. Enter viable-cell counts if you want viable-cell concentration; the tool does not classify viability or assess sample quality. Not for clinical decisions.
For educational and informational use. Not for patient care or safety-critical decisions. Check the relevant protocol and qualified guidance.
Reference and calculation checks
Thermo Fisher Scientific: Counting cells in a hemocytometer. The reference explains the underlying method; it does not endorse Toollinks.
Implementation checked October 19, 2026 using automated examples, unit conversion checks, and invalid-input checks. These checks do not certify a laboratory protocol. Read our calculation standards or report a reproducible issue to [email protected].